AllScript Transcriptase, 400 U/100 rxn

400 Units

All Script Transcriptase, Biotechrabbit
  • Skladovanie: -20°C
  • Cena bez DPH: 198,00 €
  • Cena s DPH: 217,80 €
198,00 €

AllScript Reverse Transcriptase is a proprietary RT designed for highly specific and sensitive reverse transcription. It guarantees top performance in standard reverse transcription, synthesis of ds cDNA for cloning, RT-PCR and qRT-PCR, rapid amplification of cDNA ends (RACE) or RNA analysis by primer extension. It’s high affinity to RNA allows transcription of complex RNA secondary structures and targets in low copy number, leading to high yields of cDNA.

AllScript Reverse Transcriptase is a multifunctional enzyme including RNA-dependent and ssDNA-dependent DNA polymerase, as well as RNase H activity. The RNase H activity is specific to RNA hybridized to cDNA, with no effect on pure RNA template, resulting in improved performance of subsequent PCR.

Features

  • Highly specific and sensitive RT-PCR
  • Excellent performance in transcription of complex RNA secondary structures
  • High yields of cDNA even with targets in low copy number
  • RNase H activity specific to RNA hybridized to cDNA for improved 1step PCR

Applications

  • Standard reverse transcription
  • Synthesis of ds cDNA for cloning
  • RT-PCR and qRT-PCR
  • Rapid amplification of cDNA ends (RACE)
  • RNA analysis by primer extension
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